A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Paracetamol and Flupirtine in Tablet dosage form. A YMC C18 5mm column having 250 x 4.6mm id in Isocratic mode with mobile phase containing 0.1M Potassium dihydrogen phosphate: methanol (70:30 ) was used. The flow rate was 0.8ml/min and effluents were monitored at 271nm. The method was validated for Accuracy, Precision, Specificity, Linearity and Sensitivity. The retention times of Paracetamol and Flupirtine were 1.8min and 3.0min respectively. The calibration curves were linear over a concentration range from 81.25-243.75 �µg/ml for Paracetamol and over a concentration range from 25-75 �µg/ml for Flupirtine. Limit of detection (LOD) and Limit of quantitation (LOQ) were 3.83 and 12.77 for Paracetamol and 1.05 and 3.51 for Flupirtine respectively. The developed method was fast, accurate, precise and successfully applied to estimate the amount of Paracetamol and Flupirtine in bulk sample and tablet dosage form so it can be used for regular quality control of the drug.
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